New EMBO Member’s Review Cryo-electron microscopy of vitreous sections
نویسندگان
چکیده
Ashraf Al-Amoudi, Jiin-Ju Chang, Amélie Leforestier, Alasdair McDowall, Laurée Michel Salamin, Lars PO Norlén, Karsten Richter, Nathalie Sartori Blanc, Daniel Studer and Jacques Dubochet* Laboratoire d’Analyse Ultrastructurale, Bâtiment de Biologie, Université de Lausanne, Lausanne, Switzerland, Institute of Biophysics, Academy of Sciences, Beijing, China, Laboratoire de Physique des Solides, CNRS URA002, Université Paris-Sud, Orsay, France, Centre for Microscopy and Microanalysis, University of Queensland, St Lucia, Australia, Groupe de Physique appliquée, Departement de physique, Université de Genève, Genève, Switzerland, DKFZ, Molecular Genetics (B060), Heidelberg, Germany and Anatomisches Institut, Postfach, Bern, Switzerland
منابع مشابه
Improving the technique of vitreous cryo-sectioning for cryo-electron tomography: electrostatic charging for section attachment and implementation of an anti-contamination glove box.
Cryo-electron tomography of vitreous cryo-sections is the most suitable method for exploring the 3D organization of biological samples that are too large to be imaged in an intact state. Producing good quality vitreous cryo-sections, however, is challenging. Here, we focused on the major obstacles to success: contamination in and around the microtome, and attachment of the ribbon of sections to...
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Cryo-electron microscopy has been used exclusively so far for single particle analysis or crystallography of purified proteins. Recently, technical improvements on rapid freezing and cryo-microtome permitted structural analysis of organelle or macromolecules in native state using whole cells (1,2) and vitreous cryo-sections (3). However, cryo-sections don’t scatter electrons so much and therefo...
متن کاملCloser to the native state. Critical evaluation of cryo-techniques for Transmission Electron Microscopy: preparation of biological samples.
Over the years Transmission Electron Microscopy (TEM) has evolved into a powerful technique for the structural analysis of cells and tissues at various levels of resolution. However, optimal sample preservation is required to achieve results consistent with reality. During the last few decades, conventional preparation methods have provided most of the knowledge about the ultrastructure of orga...
متن کاملAnalysis of cryo-electron microscopy images does not support the existence of 30-nm chromatin fibers in mitotic chromosomes in situ.
Although the formation of 30-nm chromatin fibers is thought to be the most basic event of chromatin compaction, it remains controversial because high-resolution imaging of chromatin in living eukaryotic cells had not been possible until now. Cryo-electron microscopy of vitreous sections is a relatively new technique, which enables direct high-resolution observation of the cell structures in a c...
متن کاملMolecular Cryo-Electron Tomography of Skin
The three-dimensional structure of skin can be studied at near-native conditions at the molecular level with a new technique called molecular electron tomography of vitreous tissue sections (tissue TOVIS). Its usage is, however, still largely limited by a difficult sample handling procedure. Here we discuss some measures for the efficient application of tissue TOVIS to dermatological research.
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تاریخ انتشار 2004